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Single-molecule and bulk biophysical approaches to study protein-DNA interactions on surface-immobilized nucleic acid templates typically rely on modifying the ends of linear DNA molecules to enable surface-DNA attachments. Unless both strands are constrained. this results in topologically free DNA molecules and the inability to observe supercoiling-dependent biological processes or r... https://www.bekindtopets.com/flash-sale-Gota-Patti-Embroidered-Chanderi-mega-choice/
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