The use of limiting dilution assay (LDA) for assessing the frequency of responders in a cell population is a method extensively used by immunologists. A series of studies addressing the statistical method of choice in an LDA have been published. However. none of these studies has addressed the point of how many wells should be employed in a given assay. https://www.redeturismobrasil.com/product-category/framed-entertainment-photos/